Fate and transport of testosterone in agricultural soils.

Hormones excreted in animal waste have been measured in surface and groundwater associated with manure that is applied to the land surface. Limited studies have been done on the fate and transport of androgenic hormones in soils. In this study, batch and column experiments were used to identify the fate and transport of radiolabeled [14C] testosterone in agricultural soils. The batch results indicated that aqueous-phase concentrations decreased for the first 5 h and then appeared to increase through time. The first-order sorption kinetics ranged from 0.08 to 0.640 h(-1) for the first 5 h. Beyond 5 h the increase in aqueous 14C could have been caused by desorption of testosterone back into the aqueous phase. However, metabolites were also produced beyond 5 h and would have likely resulted in the increase in aqueous 14C by sorption site competition and/or by lower sorption affinity. There were weak correlations of sorption with soil particle size, organic matter, and specific surface area. Testosterone was the dominant compound present in the soil column effluents, and a fully kinetic-sorption, chemical nonequilibrium model was used to describe the data. Column experiment sorption estimates were lower than the batch, which resulted from rate-limiting sorption due to the advective transport. The column degradation coefficients (0.404-0.600 h(-1)) were generally higher than values reported in the literature for 17beta-estradiol. Although it was found that testosterone degraded more readily than 17beta-estradiol, it appeared to have a greater potential to migrate in the soil because it was not as strongly sorbed. This study underlined the importance of the simultaneous transformation and sorption processes in the transport of hormones through soils.